Antioxidant enzyme profiles in a species of ornamental fish (koi)
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Date
2013-03-11
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Abstract
The aim of this work was to determine whether, and at what levels, antioxidant enzymes are expressed in the various organs of the koi. The activity of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPX), NAD(P)H: :' quinone oxidoreductase (NQOR) and malondialdehyde (MDA) content were determined in tissue homogenates of liver, kidney, pectoral muscle, gills, eggs, blood, heart and intestine. There was a marked variability (up to tenfold difference) in enzyme activity in the various organs, but much less individual variability ( - three fold difference). NQOR activity was highest in eggs (- 5.56(A/min/mg). Catalase activity also found heterogeneously in all organs had its highest activity in the liver ( - 5.02(A/min/mg). GPX (selenium dependent) activity was highest in the liver (5.14(A/min/mg). Thiobarbituric acid reactive substances (MDA) content, a measure of lipid peroxidation was, significantly low in all organs and tissues with an A535/mg of 0.00145(0.0027. The results suggest that antioxidant enzymes are expressed in most organs of the koi and that this species of fish is likely to be protected when exposed to compounds that either undergo redox cycling or that exerl a direct oxidative stress.
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Presented at the Research Day 1999.
Keywords
Antioxidant enzymes, Ornamental fish