Phenotyping of the glutathione S-transferase M1 polymorphism in Zimbabweans and the effects of chloroquine on blood glutathione S-transferases M1-A

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dc.contributor.authorMukanganyama, Stanley
dc.contributor.authorMasimirembwa, Collen M.
dc.contributor.authorNaik, Yogeshkumar S.
dc.contributor.authorHasler, Julia A.
dc.date.accessioned2015-04-09T11:27:09Z
dc.date.accessioned2023-06-23T14:00:18Z
dc.date.available2015-04-09T11:27:09Z
dc.date.available2023-06-23T14:00:18Z
dc.date.issued1997-05-31
dc.descriptionClinica Chimica Acta 265 (1997) 145-155en_US
dc.description.abstractThe frequency of the null allele phenotype of glutathione S-transferase (GST) M1 was investigated in 114 Zimbabweans and results for a subset of 63 subjects were compared with genotyping by PCR. In addition, the effect of the antimalarial chloroquine on blood levels of GSTMl and GSTA in 19 subjects was studied. Quantification of GSTs was by enzyme linked immunosorbent assays (ELISA). Thirty percent of the subjects were of the GSTMl null phenotype. Comparison of results of phenotyping by ELISA and genotyping by PCR showed that 16% of samples were in discordance; unknown mutations in the GSTMl gene in the Zimbabwean population may explain this observation. Chloroquine decreased levels of blood GSTM1 and GSTA by 50% or more. In populations treated with chloroquine, these decreases in GST activities might lead to compromised ability to detoxify xenobiotics, could confound GSTMl phenotyping and might invalidate use of GSTA as an indicator of liver damage. O 1997 Elsevier Science B.V. Keywords: Glutathione S-transferases; Phenotype; Black Zimbabweans; Chloroquineen_US
dc.description.sponsorshipThe International Program in Chemical Sciences (lpics) Uppsala University. Sweden and the University of Zimbabwe Research Board.en_US
dc.identifier.citationMukanganyama, Stanley., Masimirembwa, Collen. M.,Naik, Yoseshkumar,S. and Halser, Julia. A (1997) Phenotyping of the glutathione S-transferase M1 polymorphism in Zimbabweans and the effects of chloroquine on blood glutathione S-transferases M1-A. Elsevieren_US
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/501
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights.licenseThis article was downloaded from NUST Institutional repository, and is made available under the terms and conditions as set out in the Institutional Repository Policy.en_US
dc.subjectGlutathione S-transferasesen_US
dc.subjectPhenotype;en_US
dc.subjectBlack Zimbabweansen_US
dc.subjectChloroquineen_US
dc.titlePhenotyping of the glutathione S-transferase M1 polymorphism in Zimbabweans and the effects of chloroquine on blood glutathione S-transferases M1-Aen_US
dc.typeArticleen_US
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