Whole genome sequencing reveals insights into antibiotic resistant Klebsiella grimontii novel sequence type ST350 isolated from a wastewater source in South Africa

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dc.contributor.authorMbanga, J.
dc.contributor.authorAmoako, D.G.
dc.contributor.authorAbia, A.L.
dc.contributor.authorFatoba, D.
dc.contributor.authorEssack, S.
dc.date.accessioned2025-06-06T09:29:12Z
dc.date.available2025-06-06T09:29:12Z
dc.date.issued2022
dc.description.abstractKlebsiella grimontii is a recently identified species that has been implicated in clinical infections. Few or no reports on environmental K. grimontii exist from Africa. This study was part of a broader longitudinal research that aimed to assess the distribution, antibiotic resistance patterns, and genomics of Enterobacterales obtained from raw and treated wastewater and the associated river water of a wastewater treatment plant (WWTP) in KwaZulu-Natal, South Africa. We reported the genomics of an antibiotic resistant Klebsiella grimontii isolate obtained from the raw influent of the WWTP. Following phenotypic characterisation and antibiotic susceptibility testing, the isolate was sequenced on an Illumina MiSeq machine. Raw reads were assembled de novo by using SPAdes (v3. 6.2) prior to bioinformatics analysis. The assembled K. grimontii INF139 genome was 6,369,878 bp, with 113 contiguous sequences (> 200 bp) and 55.4% GC content. The isolate was assigned a novel sequence type ST350. Genomic analysis revealed the presence of chromosomally encoded β-lactamase (blaOXY-6-1) and fluoroquinolone (oqxB) resistance genes. Virulence factors encoding antiphagocytic, iron acquisition, and Intracellular survival properties were identified. Mobilome analysis revealed the presence of plasmid replicons (ColRNAI, FIA (pBK30683), IncFII, IncFII (Yp)) and insertion sequences (ISIX2, ISSen9, ISIS, IS1A). To our knowledge, this is the first description of antibiotic resistant K. grimontii from the water environment from Africa. The presence of this antibiotic resistant and potentially pathogenic isolate in the water environment is worrying as it may be disseminated into river systems used by informal settlements.
dc.description.sponsorshipThis work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant no. 98342), the National Research Foundation Competitive Grant for Rated Researchers (Grant no. 106063), jointly funded by the South African Medical Research Council (SAMRC) and UK Medical Research Council Newton Fund and the SAMRC Self-Initiated Research Grant.
dc.identifier.citationMbanga, J., Amoako, D.G., Abia, A.L., Fatoba, D. and Essack, S., 2022. Whole genome sequencing reveals insights into antibiotic resistant Klebsiella grimontii novel sequence type ST350 isolated from a wastewater source in South Africa. Journal of Biotech Research, 13, pp.40-45.
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/782
dc.language.isoen
dc.publisherJournal of Biotech Research
dc.titleWhole genome sequencing reveals insights into antibiotic resistant Klebsiella grimontii novel sequence type ST350 isolated from a wastewater source in South Africa
dc.typeArticle
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