Virulence factors and antibiotic resistance patterns of uropathogenic Escherichia coli

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dc.contributor.authorMbanga, Joshua
dc.contributor.authorMudzana, Raymond
dc.date.accessioned2014-12-17T09:53:21Z
dc.date.accessioned2023-06-23T14:00:28Z
dc.date.available2014-12-17T09:53:21Z
dc.date.available2023-06-23T14:00:28Z
dc.date.issued2014-10-22
dc.descriptionThis is a journal article.en_US
dc.description.abstractUrinary tract infections (UTIs)are one of the most common infections in humans and the commonest cause is Uropathogenic Escherichia coli (UPEC). UPEC possess various virulence factors which enable them to survive and grow in urine and other extra-intestinal environments. Similarly, avian pathogenic E.coli (APEC) are known for their ability to cause extra-intestinal diseases in birds. Since APEC and UPEC may encounter similar challenges when establishing infection in these locations, they may share a similar content of virulence genes and capacity to cause disease. In this study, 40 UPEC isolates were obtained from patients with suspected UTIs. Multiplex polymerase chain reaction (PCR) was then used to screen the 40 UPEC isolates for 12 virulence genes usually associated with APEC isolates. The iutA (35%), frimH (32,5%), VAT (17.5%), sitA (17.5%), sitD (15%), hlyF (12,5%), pstB (10%) and frz (7.5%) genes were detected. None of the isolates had the kpsM, ompT, unvrY and sopB genes. Antibiotic resistance patterns were also determined for all 40 isolates. A high resistance to ampicillin (90%) and tetracycline (75%) accompanied by a high sensitivity to gentamycin (82.5%) and nitrofurantoin (62.5%) was observed. Eleven multi-drug resistance patterns were observed in 65% (26/40) of the isolates. The studied UPEC isolates were shown to possess APEC associated virulence genes at low percentage frequencies suggesting a slight overlap in virulence genotypes. Antibiotic resistance patterns suggest surveillance programs to monitor drug resistance should be put in place.en_US
dc.identifier.citationMbanga, J. & Mudzana, R., 2014. Virulence factors and antibiotic resistance patterns of uropathogenic Escherichia coli. African Journal of Microbiology Research, 8(43), pp.3678–3686.en_US
dc.identifier.issn1996-0808
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/459
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.rights.licenseThis article was downloaded from NUST Institutional repository, and is made available under the terms and conditions as set out in the Institutional Repository Policy.en_US
dc.subjectUropathogenic Escherichia colien_US
dc.subjectVirulence genesen_US
dc.subjectmultiplex polymerase chain reaction (PCR)en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectZimbabween_US
dc.titleVirulence factors and antibiotic resistance patterns of uropathogenic Escherichia colien_US
dc.typeArticleen_US
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