Evaluation of test-kits for the detection of Escherichia coli O157 in raw meats and cattle faeces

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dc.contributor.authorNyati, H.
dc.contributor.authorHeuvelink, A.
dc.contributor.authorVan Heerwaarden, C.
dc.contributor.authorZwartkruis, A.
dc.date.accessioned2025-04-02T08:42:53Z
dc.date.available2025-04-02T08:42:53Z
dc.date.issued2012
dc.description.abstractEscherichia coli O157 detection limits in artificially contaminated beef and cattle faeces samples were determined using Dynabeads anti E. coli O157 immunomagnetic beads, VIDAS-UP, VIDAS-ICE, and real-time PCR (GeneDisc and LightCycler) systems. Dynabeads anti-E. coli O157 immunomagnetic separation (IMS) and the GeneDisc cycler were the most sensitive methods, and could detect an initial 1 CFU in 25g beef samples after 6h of incubation in modified tryptone soya broth with novobiocin (mTSB+n) or buffered peptone water (BPW). The VIDAS-UP method could detect an initial 10 CFU, while VIDAS-ICE and the LightCycler methods could only detect an initial 100 CFU. Higher detection rates were achieved with 18 hour incubations, where an initial 1 CFU in a 25g sample could be detected with all five methods. For cattle faeces enrichments, Dynabeads anti-E. coli O157 IMS could detect an initial 1 CFU after a 6 h incubation in mTSB+n, while the VIDAS-UP and VIDAS-ICE methods could detect an initial 10 CFU and both PCR methods could only detect an initial 100 CFU. Detection rates were lower in BPW, compared to mTSB+n, with thresholds of 100 CFU for VIDAS-ICE, VIDAS-UP and GeneDisc methods, and >100 CFU for the LightCycler method.
dc.identifier.citationNyati, H., Heuvelink, A., Van Heerwaarden, C. and Zwartkruis, A., 2012. Evaluation of test-kits for the detection of Escherichia coli O157 in raw meats and cattle faeces. International Journal of Food Studies, 1(2).
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/598
dc.language.isoen
dc.titleEvaluation of test-kits for the detection of Escherichia coli O157 in raw meats and cattle faeces
dc.typeArticle
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