Molecular identification of mycobacterium species of public health Importance in cattle in Zimbabwe by 16s rrna gene sequencing
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
BENTHAM OPEN
Abstract
Mycobacterium species are naturally found in the environment as well as in domestic animals such as cattle. So
far, more than 150 species of Mycobacterium, some of which are pathogenic, have been identified. Laboratory isolation,
detection and identification of Mycobacterium species are therefore critical if human and animal infections are to be
controlled. The objective of this study was to identify Mycobacterium species isolated in cattle in Zimbabwe using 16S
ribosomal RNA gene amplification and sequencing. A total of 134 cow dung samples were collected throughout
Zimbabwe and mycobacteria were isolated by culture. Only 49 culture isolates that were found to be acid-fast bacilli
positive by Ziehl-Neelsen staining. The 16S rRNA gene was successfully amplified by PCR in 41 (84%) of the samples.
There was no amplification in 8 (16%) of the samples. Out of the 41 samples that showed amplification, 26 (63%) had
strong PCR bands and were selected for DNA sequencing. Analysis of the DNA sequences showed that 7 (27%) belonged
to Mycobacterium neoaurum, 6 (23%) belonged to Mycobacterium fortuitum, 3 (12%) to Mycobacterium goodii, 2 (1%)
to Mycobacterium arupense, 2 (1%) to Mycobacterium peregrinum or M. septicum and 1 isolate (0.04%) to
Mycobacterium elephantis. There were 5 (19%) isolates that were non-mycobacteria and identified as Gordonia terrae, a
close relative of Mycobacterium. The study therefore provided a molecular basis for detection and identification of
Mycobacterium species in animals and humans.
Description
Keywords
Mycobacterium species, cattle, cow dung, 16S rRNA gene, sequencing
Citation
Chin’ombe, N., Muzividzi, B., Munemo, E. and Nziramasanga, P. (2016) ‘Molecular identification of Nontuberculous mycobacteria in humans in Zimbabwe using 16S Ribosequencing’, The Open Microbiology Journal, 10(1), pp. 113–123. doi: 10.2174/1874285801610010113.