Chlorpyrifos- and dichorfos-induced oxidative and neurogenic damage elicits neuro-cognitive deficits and increases anxiety-like behaviors in wild-type rats

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dc.contributor.authorImam, Aminu
dc.contributor.authorSulaiman, Nafeesah Abdulkareem
dc.contributor.authorOyewole, Aboyeji Lukuman
dc.contributor.authorChengetanai, Samson
dc.contributor.authorWilliams, Victoria
dc.contributor.authorAjibola, Musa Iyiola
dc.contributor.authorFolarin, Royhaan Olamide
dc.contributor.authorMuhammad, Asma’u Shehu
dc.contributor.authorShittu, Asma’u Shehu
dc.contributor.authorAjao, Salihu Moyosore
dc.date.accessioned2022-09-15T10:15:42Z
dc.date.accessioned2023-06-27T08:51:25Z
dc.date.available2022-09-15T10:15:42Z
dc.date.available2023-06-27T08:51:25Z
dc.date.issued2018-10-16
dc.descriptionjournal article open accessen_US
dc.description.abstractThe mechanization of agricultural activities has led to indiscriminate deposition of toxic xenobiotics, including organophosphates in the biomes, and this has led to intoxication characterized with deleterious oxidative and neuronal changes. This study investigated the consequences of oxidative and neurogenic disruptions that follow exposure to two organophosphates, chlorpyrifos (CPF) and dichlorvos (DDVP) on neuro-cognitive performance and anxiety-like behaviors in rats Thirty-two adult male Wistar rats (150 – 170g) were randomly divided into 4 groups, orally exposed to normal saline (NS), DDVP (8.8mg/kg), CPF (14.9mg/kg) and DDVP+CPF for 14 consecutive days. On day 10 of exposures, anxiety-like behaviors andamygdala dependent fear learning were assessed using Open Field and Elevated Plus Maze paradigms respectively, while spatial working memory was assessed on day 14 in the Morris water maze paradigm, following 3 training trials each on days 11, 12 and 13. On day 15, the rats were euthanized, and their brains excised, hippocampus and amygdala removed, 5 of which were homogenized and centrifuged to analyze nitric oxide (NO) metabolites, total reactive oxygen species (ROS), and acetylcholinesterase (AChE) activity, and the other three processed for histology 43 (cresyl violet stain) and proliferative marker (Ki67 immunohistochemistry). Marked (p≤0.05) loss in 44 body weight, AChE depletion, and overproduction of both NO and ROS were observed after repeated exposure to individual and combined doses of CPF and DDVP. Insults from DDVP exposure appeared more severe owing to the observed greater losses in the body weights of exposed rats. There was also a significant (p≤0.05) effect on the cognitive behaviors recorded from the exposed rats, and these deficits were related to the oxidative damage and neurogenic cell loss in the hippocampus and the amygdala of the exposed rats. Taken together, these results provided an Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 16 October 2018 doi:10.20944/preprints201810.0359.v1 © 2018 by the author(s). Distributed under a Creative Commons CC BY license.2 of 16 insight that oxidative and neurogenic damages are central to the severity of neuro-cognitive dysfunction and increased anxiety-like behaviors that follow organophosphate poisoningen_US
dc.description.sponsorshipDeanship Research support of the Faculty of Basic Medical Sciences, Ms. Ali Hussain of the Immunohistochemistry and histology laboratory, School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, South Africa.en_US
dc.identifier.citationImam A, and Sulaiman N.A.et.al.(2018). Chlorpyrifos- and dichorfos-induced oxidative and neurogenic damage elicits neuro-cognitive deficits and increases anxiety-like behaviors in wild-type rats.1,1-16p.en_US
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/992
dc.language.isoenen_US
dc.publisherCreative commonsen_US
dc.subjectanxiety-like behaviors in wild-type ratsen_US
dc.subjectChlorpyrifosen_US
dc.titleChlorpyrifos- and dichorfos-induced oxidative and neurogenic damage elicits neuro-cognitive deficits and increases anxiety-like behaviors in wild-type ratsen_US
dc.typeArticleen_US
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