Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
International Organization Of Scientific Research (IOSR).
Abstract
The study sought to characterize Fasciola gigantica isolates from cattle in different localities using
RAPD-PCR. Adult flukes morphologically identified as F. gigantica were collected from slaughtered infected
animals during meat hygiene inspections. DNA was extracted from single flukes and subjected to RAPD-PCR
analysis. In the RAPD-PCR analysis, genomic DNA isolated from the conical anterior end of the worms was
amplified by the polymerase chain reaction using 10 random oligonucleotide primers. Depending upon the
Fasciola gigantica isolate-primer combination, 1-13 DNA fragments in the range of 75-2000bp were amplified.
It was observed that all the 10 primers directing amplification of DNA were of potential interest in the
generation of polymorphic DNA. The percentage polymorphic loci ranged from 33.33-100%. Polymorphic
bands were scored and used to calculate Nei’s 1978 genetic distance. The genetic distance values ranged
between 0.0690 (isolate 5 and 6 from Gwanda and 0.6109 (isolate 6 from Gwanda and isolate 14 from Matopo).
The mean Nei’s gene diversity was 0.2839. The study showed the variability of Fasciola gigantica isolates from
the same host, using RAPD markers could be applied as a low cost way of identification
Description
This ia n journal article in the IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS), published by International Organization Of Scientific Research (IOSR)in 2014. It is focusing on the characterisation of fasciola gigantica isolates from cattle using RAPD-RCR. The study is based in Zimbabwe's Western region.
Keywords
Cattle, Fasciola, morphology, RAPD-PCR, Zimbabwe
Citation
Chauke, E. et al. 2014. Characterization of Fasciola gigantica isolates from cattle from South-Western Zimbabwe using RAPD-PCR. IOSR Journal of Agriculture and Veterinary Science, Vol 7, Issue. 2, Ver. I,pp. 19-25.