Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR

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dc.contributor.authorChauke, E.
dc.contributor.authorDhlamini, Zephaniah
dc.contributor.authorMbanga, Joshua
dc.contributor.authorDube, S.
dc.date.accessioned2014-12-18T06:51:52Z
dc.date.accessioned2023-06-23T14:00:33Z
dc.date.available2014-12-18T06:51:52Z
dc.date.available2023-06-23T14:00:33Z
dc.date.issued2014
dc.descriptionThis ia n journal article in the IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS), published by International Organization Of Scientific Research (IOSR)in 2014. It is focusing on the characterisation of fasciola gigantica isolates from cattle using RAPD-RCR. The study is based in Zimbabwe's Western region.en_US
dc.description.abstractThe study sought to characterize Fasciola gigantica isolates from cattle in different localities using RAPD-PCR. Adult flukes morphologically identified as F. gigantica were collected from slaughtered infected animals during meat hygiene inspections. DNA was extracted from single flukes and subjected to RAPD-PCR analysis. In the RAPD-PCR analysis, genomic DNA isolated from the conical anterior end of the worms was amplified by the polymerase chain reaction using 10 random oligonucleotide primers. Depending upon the Fasciola gigantica isolate-primer combination, 1-13 DNA fragments in the range of 75-2000bp were amplified. It was observed that all the 10 primers directing amplification of DNA were of potential interest in the generation of polymorphic DNA. The percentage polymorphic loci ranged from 33.33-100%. Polymorphic bands were scored and used to calculate Nei’s 1978 genetic distance. The genetic distance values ranged between 0.0690 (isolate 5 and 6 from Gwanda and 0.6109 (isolate 6 from Gwanda and isolate 14 from Matopo). The mean Nei’s gene diversity was 0.2839. The study showed the variability of Fasciola gigantica isolates from the same host, using RAPD markers could be applied as a low cost way of identificationen_US
dc.identifier.citationChauke, E. et al. 2014. Characterization of Fasciola gigantica isolates from cattle from South-Western Zimbabwe using RAPD-PCR. IOSR Journal of Agriculture and Veterinary Science, Vol 7, Issue. 2, Ver. I,pp. 19-25.en_US
dc.identifier.issn2319-2380
dc.identifier.urihttp://196.220.97.103:4000/handle/123456789/470
dc.language.isoenen_US
dc.publisherInternational Organization Of Scientific Research (IOSR).en_US
dc.rights.licenseThis article was downloaded from NUST Institutional repository, and is made available under the terms and conditions as set out in the Institutional Repository Policy.en_US
dc.subjectCattleen_US
dc.subjectFasciolaen_US
dc.subjectmorphologyen_US
dc.subjectRAPD-PCRen_US
dc.subjectZimbabween_US
dc.titleCharacterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCRen_US
dc.typeArticleen_US
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