Effects of chloroquine treatment on antioxidant enzymes in rat liver and kidney.
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Date
1996-05-09
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Inc.
Abstract
The effect of chloroquine (CHQ) administration on antioxidant enzymes in rat liver and kidney was
studied. Male Sprague-Dawley rats were administered 20 mglkg CHQ once a week for 4 weeks (chronic treatment)
or a single dose at 10 or 20 mglkg (acute treatment). Antioxidant enzyme activities were determined in cytosolic
fractions of liver and kidney, whereas reduced glutathione (GSH) and malondialdehyde (MDA) were determined in
tissue samples. Results indicate minimal effects of acute CHQ treatment, whereas chronic treatment with CHQ
differentially affected antioxidant enzymes in the two organs. Superoxide dismutase activity was increased nearly
twofold, while activities of selenium glutathione peroxidase (GPX), catalase, and NAD (P) H: quinone oxidoreductase
were decreased in livers of CHQ-treated rats compared to controls. No significant effects of CHQ on glutathione
reductase, GSH, and MDA levels were seen in the liver. Fewer effects of CHQ were observed in the kidney where
a decrease in GPX activity and an increase in MDA levels was seen. Lowering of antioxidant enzymes activities in
the liver by CHQ could render the organ more susceptible to subsequent oxidative stress; while increased MDA
production after CHQ treatment in the kidney indicate that the organ is being subjected to oxidative stress. This
could have implications for prolonged chloroquine intake.
Description
A journal article with illustrations
Keywords
Antioxidant enzymes, Chloroquine, Oxidative stress, Liver, Kidney, Rats, Free radicals
Citation
Magwere T, Naik Y.S, Hasler A. J. (1996) Effects of chloroquine treatment on antioxidant enzymes in rat liver and kidney.Elsevier Inc.