Xenobiotic metabolizing enzymes of the freshwater snails.Planorbella and lymnaea (RADIX)Natalensis.
Loading...
Date
1994
Journal Title
Journal ISSN
Volume Title
Publisher
J. Med. and Appl. Malacol
Abstract
Very little is known about the ability of snails to metabolise and remove xenobiotics such as molluscicides. The present study was conducted to determine whether the
freshwater snails Planorbella duryi and Lymnaea (Radix) nntaIensis possess two of the
major enzyme systems of detoxication, namely the P450 monooxygenase system and the
glutathione Stransferases. We were able to measure microsomal cytochrome b5 and
NADPH cytochrome c reductase activity, indicating a functional mixed function oxidase
system. However, probing with three known substrates for the mammalian enzyme, we
were unable to detect any cytochrome P450 mediated activity. Glutathione Stransferase
activity was detectable in cytosolic fractions. In general, the activities detectable in snails
were much lower than in mammalian liver preparations. The existence of these enzymes
in snails suggests that studies should be undertaken to observe the interaction between
these enzymes and xenobiotics such as candidate molluscicides.
Key words: Planorbella duyi, Lyrnnaea (Radix) natalensis, cytochrome P450, glutathione
Description
Journal of medical and applie macology
Keywords
Planorbella duyi, Lyrnnaea (Radix) natalensis, cytochrome P450, Glutathione S-transferase
Citation
Naik, Y.S., Magwere, M.,Matiyenga, M and Halser, J.A (1994) Xenobitic metabolizing enzymes of the freshwater snails.Planorbella and lymnaea (Radix) Natalensis.